Cell Culture Established from Warts of Bovine Papilloma

This study is considered the first in the country on growing of bovine papilloma in cell culture from skin warts of cattle in order to detect papilloma virus, and to establisha cell line of transformed cells for further studies. In this study the warts were surgically removed from cows showing lump lesions on skin of abdomen, neck and udder, and transferred aseptically to laboratory by transport media. Trypsin and collagenase enzyme were used to dispersed papilloma cells from fragments of warts, Dulbecco’s modified Eagle’s medium (DMEM) and Rosswell Park Memorial Institute (RPMI)-1640 Medium (Gibco, were used with 10% fetal calf serum for culturing of papilloma cells. Cultured cell were first noticed after 3-4 days post incubation(PI) and appeared as epithelial and fibroblastic cells. After 7days post inoculation clones oftransformed epithelial cells start to appear and after (20-25) days it became a complete monolayer. Successful secondary culture was achieved by using trypsin-versene solution(TV). Further studies is needed to detect the virus from cultured cell by PCR and ELISA techniques beside preparation of vaccine from cell culture for treatment of papillomas in infected cattle.