Prolactin, ICSH and Testosterone Blood Levels in Hemi castrated 8-10 Weeks old kids

Eighteen (18) 8-10 weeks old kid were used to study the age of puberty. Surgical hemi castration for 10 kids at age 8-10 weeks was done for left testis using local anesthesia. Tissue and swaps samples form testis and epididymis were taken to determined sperms present. At age of 14-16 weeks five hemicastrated kids and four normal kids were injected with 5 doses of 10 μg GnRH I/M injected with an intervals of 48 hrs, blood samples were taken to asses the ICSH, testosterone and prolactin plasma level after first surgical and GnRH injection. Drops from seminal fluids were taken directly on glass slides and stained to record the presences of sperms. At age of 18-20 weeks second surgical operation for the right testis was done for 6 males from different 4 groups. Tissue and smear samples were, also taken to determine the sperm presence. The results were showed there were no sperm recorded in samples at 8-10 weeks kids. After hemi castrated and GnRH treated kids showed early sex behavior and number of spermatozoa were found with drops of fluid secreted from penis at around age of 14-16 months. Also the swaps and testes tissues were showed presences of sperms. The results of 16-18 weeks old hemi castrated and GnRH injections kids were showed increased (P 0.05) in levels of ICSH and testosterone. After second removed of one or both testes the control kids had significant effects (P 0.05) for ICSH and prolactin hormones and (P 0.01) testosterone Present study concluded, it can be initiate the early puberty stage by removing one testis and or GnRH injection of Iraqi kids at age of 14-16 weeks.