Propagation of bovine parainfluenza type 3 virus in chicken embryos A. S. Al-Bana and A. B. Allawe

Bovine parainfluenza type 3 virus (BPIV-3) was first isolated in secondary embryonic bovine kidney cell culture (SEBK), and then the virus was propagated for the first time in chicken embryos by allantoic inoculation method. The virus was propagated for four passages in chicken embryos, then the propagated virus was inoculated in SEBK cell culture and cytopathic effects of inoculated virus (focal rounding of cells with syncytia) were noticed. Heamagglutination test was used to detect the virus in harvested allantoic fluid with 0.5% guinea pig RBCs. The virus was diagnosed by heamagglutination inhibition test with 0.5% guinea pig RBCs by using standard antiserum for BPIV-3. Reverse-transcriptase polymerase chain reaction (RTPCR) was used for detection of propagated BPIV-3 in chicken embryos allantoic fluids