Author/Authors :
Mohammed, Sh. A. Tikrit University - Education College, Iraq , Abudulla, A. H. Tikrit University - Education College, Iraq , Buniya, H. K. University of Anbar - College of Education for Pure Science, Iraq , Mkhlaf, M. M. University of Anbar - College of Education for Pure Science, Iraq
Abstract :
In this study, 27 married patients were totally examined for the presence of antibodies against Toxoplasma gondii by using a standard commercial enzyme-linking immunosorbent assay (ELISA) and Polymerase Chain Reactin (PCR) technique was used evaluate the genome of this parasite. Of 27 whole blood of married samples, nested PCR was positive in 22 (77.77%) by got the 96 bp DNA fragment, 9(40.9%) of them appeared with IgM and 6 (27.27%) of no anti-Toxoplasma, while 7 (31.81%) was positive nPCR results revealed both IgM+ and IgG+ antibodies, and 5 (22.22%) were negative results obtained by ELISA and nPCR.
