UJI PENGHAMBATAN AKTIVITAS α-GLUKOSIDASE EKSTRAK DAN FRAKSI DAUN Antidesma neurocarpum Miq.

Diabetes mellitus is a metabolic dysfunction disease showed by hyperglicemia and abnormality of carbohydrates, fats, and proteins metabolism. It is known can be cured with a-glucosidase inhibitor. Previous experiment reported that methanol extract from leaves of Antidesma neurocarpum Miq. has a strong inhibitory activity of a-glucosidase (IC50=2.18 μg/mL). The aim of this experiment was to know about the most active extract and fraction of inhibitory activity of a-glycosidase and also the chemical compounds from those most active extract and fraction. Extraction is done by using multilevel maceration (n-hexane, ethyl acetate and methanol). Inhibitory activity of a-glucosidase tested with microplate reader at 405 nm wavelength. As standard, we used acarbose (IC50=38.37 μg/mL). Methanol extract has the strongest inhibitory activity of a-glucosidase as shown the highest inhibitor percentage (89.53%). It is identified the chemical compounds and fracinationed by flash column chromatography. Fractination produced 15 combined fractions. Combined fractions which more than 200 mg tested to know their inhibitory activity of a-glucosidase. Result showed that the most active fraction is the 8th (IC50=40.77μg/mL) with a competitive inhibitor mechanism to a-glycosidase. Chemical compounds that is found in the methanol extract and 8th fraction of Antidesma neurocarpum Miq. leaves areflavonoids, tannins, glycosides, fenol and alkaloids