The effect of Brucella melitensis (Rev-1)and sonicated antigen of Salmonella typhimurium on some interlukins (IL-4, IL-6) and IgM in rabbits

The research is designed to prepare antigens of Brucella melitensis and sonicated Salmonella typhimurium and their synergistic effect on some interlukines (IL-4,IL-6) and Immunoglobulin-M (IgM) which were evaluated by using enzyme linked immunosorbant assay ( ELISA). For this purpose 25 Rabbits randomly divided into five groups each group contained five animals. The first group was immunized with Brucella melitensis (rev-1) (1-2 * 10^9) cfu/ml and sonicated Salmonella typhimurium 1000 μg/ml, Subcutaneously. The 2nd group was immunized with. B. melitensis (rev-1) 1-2 * 10^9 cfu/ml and sonicated S. typhimurium 500 μg/ml Subcutaneously. The 3rd group was immunized with B. melitensis (rev-1) (1-2 * 10^9) cfu/ml. The 4th group was immunized with sonicated S. typhimurium1000 μg/ml only Subcutaneously as positive control. The 5th group was immunized by P.B.S.(pH7.2) as negative control group. The high concentration of IL-4,IL6 was recorded in the first group, It was (177.54±2.25 and 252.35±13.35), respectively and the lowest concentration was in the fourth group (144.32±4.85 and 119.78±3.34), respectively. Also the results of IgM showed that the highest concentration was 501.23±41.22 in the first group, while the lowest concentration was 63.99±8.76 in the fourth group. Apparent differences in the levels of IL-6, IL-4 and IgM-class antibody in the groups injected with Killed whole cell sonicated antigen of Salmonella typhimurium (KWCSA-ST1000 mg/ml) and B. melitensis (rev-1) (1-2 * 10^9) cfu/ml and the group injected with KWCSA-ST (500 mg/ml) and B. melitensis (rev-1) (1-2 * 10^9) cfu/ml as compared with the other groups which injected with KWCSA-ST(1000 mg/ml) only and B. melitensis (rev-1) (1-2 * 10^9) cfu/ml only