Sporicidal Activity of some Disinfectants Against Clostridium Perfringens Isolated from Broiler Poultry Litter

Necrotic enteritis and the subclinical form of Clostridium perfringens infection in poultry are caused by C. perfringens type A, producing the alpha toxin, and to a lesser extent type C, producing both alpha toxin and beta toxin C and have become serious threats to poultry health.This study was undertaken to determine the activity of 4 commonly available disinfectants against C. perfringens spores after 5,7.5,10,15,30 and 60 minutes of contact under dirty conditions, Of the 4 products tested, calcium hypochlorite 1% and Germicidan KOK 4% achieved the required reduction in microbial viability (≥10³-fold) for relatively long contact times of 30 min, under dirty (3% yeast) conditions, One product (Biosentury 904, 2% conc) achieved the required reduction in microbial viability (≥10³-fold) for contact times of 10 minutes, while Prophyl 75,1% concentration. achieved the required reduction in microbial viability (≥10³-fold) for contact times of 5minutes.Upon addition of formic acid 2% and urea 1% the required reduction improved for (Biosentury 904, 2% conc) and Prophyl 75,1% concentration and achieved after 5 minutes and after 7.5 minutes for Germicidan KOK 4% while calcium hypochlorite was not improved by addition of formic acid 2% or urea 1%. Application of surface test using the four disinfectants was used alone and in combination with formic acid 2% or urea 1% showed nearly the same results obtained in the suspension test.