Author/Authors :
YUSOFF, N.MOHD. International Islamic University Malaysia - Bioprocess and Molecular Engineering Research Unit,Department of Biotechnology Engineering, Malaysia , NUGE, T. International Islamic University Malaysia - Bioprocess and Molecular Engineering Research Unit,Department of Biotechnology Engineering, Malaysia , ZAINAN, N.H. International Islamic University Malaysia - Bioprocess and Molecular Engineering Research Unit,Department of Biotechnology Engineering, Malaysia , HASHIM, Y.Z.H-Y. International Islamic University Malaysia - Bioprocess and Molecular Engineering Research Unit,Department of Biotechnology Engineering, Malaysia , JAMAL, P. International Islamic University Malaysia - Bioprocess and Molecular Engineering Research Unit,Department of Biotechnology Engineering, Malaysia , HUSSIN, ANIS SHOBIRIN MEOR Universiti Putra Malaysia - Faculty of Science and Food Technology - Department of Food Technology, Malaysia , FAROUK, ABD-ELAZIEM Taif University - Faculty of Science - Department of Biotechnology, Saudi Arabia , SALLEH, H.M. International Islamic University Malaysia - Bioprocess and Molecular Engineering Research Unit,Department of Biotechnology Engineering, Malaysia
Abstract :
Phytate or myo-inositol hexakisphosphates (IP_6) is widely distributed in plants like rice bran. The production of myo-inositol phosphate intermediates has received much attention due to the remarkable potential health benefits offered by the compounds. In this study, the cytotoxicity of the partially purified myo-inositol phosphate fractions and commercial IP_1 and IP_6 were investigated against MCF-7 breast cancer cell lines. The study showed that the commercial standard IP1 and IP6 showed good inhibition towards the MCF-7 cell line. The MCF-7 cells growth was inhibited in minimum concentration of myo-inositol phosphates ( 1000 μg/ml). However, no inhibition observed on the MCF-7 cell line by the myo-inositol phosphates fractions partially purified from rice bran at concentration 1000 μg/ml. The inhibition of MCF-7 was only observed at concentration more than 30 mg/ml with more than 40% cells were inhibited. This indicates that the partially purified rice bran myo-inositol phosphates degraded by ASUIA279 phytase on MCF-7 breast cancer cells exhibit positive results towards the inhibition of cancer cells growth at relatively high concentration.
Keywords :
myo , inositol phosphates , phytase , MCF , 7cell line , cancer
