Title of article
Genetic Polymorphism between Natural Populations of the Nile Tilapia (Oreochromis niloticus L.) Based on Randomly Amplified Polymorphie DNA Markers
Author/Authors
khaled, asmaa a. alexandria university - faculty of agriculture - animal and fish production department, Alexandria, Alexandria
From page
698
To page
702
Abstract
Nine natural population of Nile Tilapia (Oreochromis niloticus L.,) were collected from different governorates in Egypt to calculate the genetic polymorphism with/within the different populations. Collected fish samples were subjected to DNA extraction using arbitrary short (10 nucleotides) primers randomly amplified polymorphie DNA -PCR. In a total of 726 amplification fragments; 430 were polymorphic and 296 were monomorphic with general genetic polymorphism percentage 59.23%. The recorded results indicated that there were high significant variations regarding the population from different location in Egypt. The genetic polymorphism in the differentpopulations were (56.84%) in Marsa Matroh governorate: Siwa oasis (54.43%) and Al-Negelia (59.25%); (60.08%) in Alexandria governorate: Abis (59.34%), Alameryia (60.82%); (59.26%) in El-Beheria governorate: Nobariya (59.21%), Edko (58.02%), Rosetta (60.56%); Kafer Elshikh governorate: Desuq (59.64%) and 59.74% in Aswan governorate. The highest amplification fragments were recorded to OPN-10 (98), OPQ-14 (90) and OPM-5 (86) primers, in respect.
Keywords
Genetic polymorphism , populations , Nile tilapia , RAPD
Journal title
alexandria science exchange journal
Journal title
alexandria science exchange journal
Record number
2656495
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