Author/Authors :
Siti Syarifah, MM Forest Research Institute Malaysia, Malaysia , Nurhanan, MY Forest Research Institute Malaysia, Malaysia , Muhd Haffiz, J Forest Research Institute Malaysia, Malaysia , Mohd Ilham, A Forest Research Institute Malaysia, Malaysia , Mohd Ilham, A Ministry of Science, Technology and Innovation - Malaysian Institute of Pharmaceuticals and Nutraceuticals, Malaysia , Getha, K Forest Research Institute Malaysia, Malaysia , Asiah, O Forest Research Institute Malaysia, Malaysia , Norhayati, I Forest Research Institute Malaysia, Malaysia , Lili Sahira, H Forest Research Institute Malaysia, Malaysia , Anee Suryani, S Forest Research Institute Malaysia, Malaysia
Abstract :
The cytotoxicity of the leaf of Cerbera odollam was investigated against two breast cancer cell lines (T47D and MCF7), two ovarian cancer cell lines (SKOV3 and Caov3) and a normal (Vero) cell line. The crude methanolic extract was subjected to liquid–liquid fractionation with ethyl acetate, butanol and water. Ethyl acetate fraction exhibited higher cytotoxic effect than methanol, butanol and aqueous fractions. Following bioassay guided isolation, 17βH-neriifolin was isolated as a potential anticancer agent from C. odollam leaf. It showed potent anticancer activity with IC50 values of 17, 21, 28, 32 and 24 nM against MCF7, T47D, SKOV3, CaOV3 and Vero cell lines respectively.
Keywords :
Ovarian cancer , breast cancer , plant extracts , carcinoma cell lines , SRB assay
