Electrochemical Detection of Celestine Blue Based on Screen Printed Carbon Electrode Modified with Molecular Imprinted Polymer and NiO Nanoparticles

A simple and rapid electrochemical method was developed for the detection of trace amount of Celestine blue (CB) at the surface of modified screen-printed carbon electrode (SPCE) with nickel oxide (NiO) nanoparticles and molecular imprinted polymer (MIP). Various types of electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were employed to probe the characteristics of the sensor toward CB. The selectivity, real sample analysis (efficiency), the linear concentration range, and the detection limit of CB at the MIP/NiO-NPs/SPCE were evaluated by DPV method. FTIR spectroscopy was applied to characterize the synthesized CB-MIP and non-molecular imprinted polymers (NIP). Scanning electron microscopy (SEM) and BET (Brunauer-Emmett-Teller) techniques were used for surface morphology and porosity properties of CB-MIP and NIP. A linear range was presented by the sensor for CB concentration between 2-150 μM with detection limit (S/N = 3) of 0.35 μM. The sensor is able to selectively detect the CB molecules over the potential interferences. The sensor was used to determine CB in the real sample with acceptable results.