Author/Authors :
Mossahebimohammadi, Majid tarbiat modares university - Department of Hematology and Blood banking, تهران, ايران , Kaviani, Saeid tarbiat modares university - Faculty of Medical Sciences - Department of Hematology and Blood banking, تهران, ايران , Soufizomorrod, Mina tarbiat modares university - Department of Hematology and Blood banking, تهران, ايران , Soleimani, Masoud tarbiat modares university - Department of Hematology and Blood banking, تهران, ايران , Abroun, Saeid tarbiat modares university - Department of Hematology and Blood banking, تهران, ايران , Khamisipour, Gholamreza bushehr university of medical sciences - Department of Allied Medicine, ايران , Saki, Najmaldin ahvaz jundishapur university of medical sciences - Research Center of Thalassemia Hemoglobinopathy, اهواز, ايران
Abstract :
Embryonic stem cells (ESCs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. Also, these cells have the self-renewing ability. ESCs could differentiate into most of cell lineages. Lymphocytes generated from hematopoietic stem cells. We designed a feeder free culture condition to generate lymphoid lineage cells from ESCs. We demonstrated that IL-7 plays critical role in differentiation of ESCs into lymphoid lineage and expression of specific cell markers such as CD3, CD19 and CD25 after 14 days of differentiation by RT-PCR.
Keywords :
Genes Expression , Mouse Embryonic Stem Cells , Differentiation , Feeder Free Condition
