STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY

The effect of some compounds and ions such as EDTA,Urea,KCN,2-Mercaptoethanol,BeCl2 and L-Phenyl alanine on the activity of Alkaline phosphatase extracted from Escherichia coli has been studied by using p-NitroPhenyl Phosphate as a substrate at a different concentrations (1.5×10-1,3.0×10-2,4.5×10-3) M.,and the alkaline glycine regulating solution pH=10.5.Inhibition constant Ki and type of inhibition(competitive or noncompetitive)using Dixon plot for competitive noncompetitive inhibition as well as the percentage of inhibition have all been calculated I50 for different compounds