a simple method for extraction and purification of hypericins from st john s wort

hypericin was extracted from hypericum perforatum l. using a different extraction procedure. highest amounts of hypericins were extracted by ultrasonic methods using methanol: acetone 2:1 as an extracting solvent. hypericin contents of the extract was purified by passing it through 2 series of 70×5 mm glass columns, each filled with 800 mg of 35 - 70 mesh silica gel, followed by elution with meoh: acetone: ch2cl2 (75:10:15). the extent of purification was determined by hplc. the amount of purified hypericins, which was extracted from hypericum perforatum, was 5.105 mg per gram dried plant leaves.