Title of article :
improved system for constructing bacterial cdna libraries from the venom glands of two iranian scorpions
Author/Authors :
naderi soorki, maryam shahid chamran university of ahvaz - faculty of science - department of genetics, ايران , galehdari, hamid shahid chamran university of ahvaz - faculty of science - department of genetics, ايران , jalali, amir ahvaz jundishapur university of medical sciences - school of pharmacy,toxicology research center - department of pharmacology and toxicology, ايران , baradaran, masoumeh ahvaz jundishapur university of medical sciences - toxicology research center, ايران
From page :
1
To page :
6
Abstract :
background: analyzing a cell or a tissue transcriptome is the best way to understand its functions. cdna library construction is one of the best methods to achieve this aim, and it is used to determine the structure, functions, and expression profile of a transcriptome. in the construction of a cdna library, bacterial hosts are very adaptive. one of the most adaptive bacterial hosts is the dh5α strain of escherichia coli. objectives: the venom peptides of scorpions are applicable in biological and medical research as potent drugs, as they have bioac- tive components; therefore, their identification is very important. in this project, we set up and improved the clontech® in-fusion smarter™ directional cdna library construction kit in the transcriptome analysis of the venom glands of two iranian scorpions, odonthubuthus doriae (o. doriae) and mesobuthus eupeus (m. eupeus). methods: rna extraction and cdna synthesis and purification were conducted on the venom glands of o. doriae and m. eupeus scor- pions. cdna libraries were constructed by cloning and transforming chemically competent bacterial cells. the blue-white screen method was used to identify the transformed cells. for finding colonies that have scorpion-related inserts, a colony pcr method was designed with specific primers for two sides of the insertion site into the vector. results: the obtained white colonies have vectors that include cdna related to o. doriae and m. eupeus. conclusions: the constructed cdna libraries of o. doriae and m. eupeus glands confirmed that a framework was created for the transcriptome analysis and peptide study of the venom glands of other scorpion species.
Keywords :
kit improvement , cdna library construction , iranian scorpion venom gland
Journal title :
Jundishapur Journal of Natural Pharmaceutical Products (JJNPP)
Journal title :
Jundishapur Journal of Natural Pharmaceutical Products (JJNPP)
Record number :
2663412
Link To Document :
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