Title of article
Separation of Tocol-Derivatives by Elevated Temperature Normal-Phase Liquid Chromatography
Author/Authors
SANAGI, MOHD MARSIN Universiti Teknologi Malaysia - Faculty of Science - Department of Chemistry, Malaysia , See, Hong Heng Universiti Teknologi Malaysia - Faculty of Science - Department of Chemistry, Malaysia , IBRAHIM, WAN AINI WAN Universiti Teknologi Malaysia - Faculty of Science - Department of Chemistry, Malaysia , ABU NAIM, AHMEDY Universiti Teknologi Malaysia - Faculty of Science - Department of Chemistry, Malaysia
From page
29
To page
40
Abstract
A novel method for the separation of eight vitamin E isomers (alpha,beta,Upsilon,deltatocopherol), and alpha,beta,Upsilon,delta-tocotrienol) and alpha-tocopherol acetate on normal phase high performance liquid chromatography (NP-HPLC) at elevated column temperature has been developed. The separation was optimized by varying the eluent composition and column temperature. All the isomers were successfully separated using NP-HPLC on amino and silica columns. By simply increasing the temperature for silica column, excellent separation efficiencies and shorter analysis times were achieved without significant loss in resolution. The developed separation method is rapid, shows excellent reproducibility, and suitable to be used as a quantitative method in analyzing tocopherols and tocotrienols.
Keywords
Elevated temperature HPLC , tocopherols , tocotrienols , vitamin E
Journal title
Jurnal Teknologi :C
Journal title
Jurnal Teknologi :C
Record number
2666189
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