Author/Authors :
Azam Sofi, Department of Range and Watershed Management - Soil and Water Engineering Faculty - University of Zabol - Zabol, Iran , Ebrahimi, Mahdieh Department of Range and Watershed Management - Soil and Water Engineering Faculty - University of Zabol - Zabol, Iran , Shirmohammadi, Ebrahim Department of Soil Engineering - Soil and Water Engineering Faculty - University of Zabol - Zabol, Iran
Abstract :
The study was done to determine the effect of humic acid on germination, morphological
properties, and photosynthesis pigments of Trifolium alexandrium L. under salt stress.
Methods: This study was conducted in a as a completely randomized design was humic acid
(0,0.009mg Li−1). The second factor was salt stress (0,0.75,1.5,3dSm−1). Seed germination,
vigor index, allometric coefficient, radicle and pedicle length, totally dry and fresh weight, and
photosynthetic contents were measured.
Findings: The minimum and maximum germination rate (4.00 and 4.90seed/day) and
vigor index (1.79 and 6.33) were related to 3dSm-1 and H+3dSm-1 treatments, respectively.
The germination percentage did not show a significant difference (p<0.05). The radicle and
pedicle lengths, allometric coefficient, germination rate, and vigor index in the zero treatment
were more than the H treatment, but they decreased with increasing salinity. The minimum
and maximum radicle (0.79 and 2.30cm), pedicle lengths (0.79 and 2.55cm) and dry weight
(0.0014 and 0.0026g) were related to the 3dSm-1 and H+3dSm-1 treatments, respectively. The
highest chlorophyll a (0.31 mg g-1 fresh weight), carotenoid (0.07 mg. g-1 fresh weight) and
total chlorophyll (0.48mg. g-1 fresh weight) were related to H+1.5dS. m-1. The highest amount
of chlorophyll b (0.34mg. g-1 fresh weight) was observed in H+3dS. m-1.
Conclusion: Humic acid had different but positive effects on the plant’s quantitative
properties and germination characteristics in salinity stress. The use of this fertilizer in
salinity stress for salinity-sensitive plants can be helpful, but a more comprehensive survey
in the field is recommended.
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Keywords :
total chlorophyll , humic substances , seedling vigor index , Abiotic stress
