Author/Authors :
MAN, ROHAIDA CHE Universiti Malaysia Pahang - Faculty of Chemical Engineering Natural Resources, Malaysia , HASSAN, OSMAN Universiti Kebangsaan Malaysia - School of Chemistry and Food Technology, Faculty Science and Technology, Malaysia , ABDUL MURAD, ABDUL MUNIR Universiti Kebangsaan Malaysia - School of Bioscience and Biotechnology, Faculty Science and Technology, Malaysia , ABU BAKAR, FARAH DIBA Universiti Kebangsaan Malaysia - School of Bioscience and Biotechnology, Faculty Science and Technology, Malaysia , CHARLIE, AGNESE JATI Universiti Teknologi Malaysia - Faculty of Chemical Engineering and Natural Resources Engineering - Department of Bioprocess, Malaysia , lLLIAS, ROSULI MD Universiti Teknologi Malaysia - Faculty of Chemical Engineering and Natural Resources Engineering - Department of Bioprocess, Malaysia
Abstract :
The effects of different mediums, glucose, lactose and DPTG on production of chitinase from Trichoderma virens UKM-1 in Escherichia coli were investigated in this study. Five different medium were tested namely LB, 2xYT, TB, MTB and SOB. The results indicated that expression was highest using LB medium. Further improvement of chitinase production was carried out by the addition and manipulating of glucose, lactose and IPTG content using response surface methodology by implementing the central composite design. Response surface analysis revealed that the optimum values of the tested variables for the production of chitinase were: glucose 0.2%, lactose 0.5% and IPTG 1.25 mM. The optimal production of recombinant chitinase achieved was 0.675 U/ml. This shows 1.2-fold increment compared to former optimized cultural condition which produced 0.561 U/ml of chitinase activity
Keywords :
Chitinase , production , trichoderma virens UKM , 1 , response surface methodology , chitinase activity
