Author/Authors :
MOBARAK, HANAN A. Cairo University - Faculty of Medicine - Department of Physiology, Egypt , AMMAR, HANIA I. Cairo University - Faculty of Medicine - Department of Physiology, Egypt , SEDDIEK, HANAN A. Cairo University - Faculty of Medicine - Department of Physiology, Egypt , DARWISH, AMAL S. Cairo University - Faculty of Medicine - Department of Obstetrics and Gynecology, Egypt
Abstract :
Aim of the Work: It has been proposed that preeclampsia is a disorder of increased oxidative stress. offering the possibility of targeted therapy aimed at oxidative stress reduction with antioxidants. The present work was done to study the benejicial effec t of antioxidant supplementation on prevention of preeclampsia in rats with pregnancy induced hyp ertension. Methods: Ninety fe male pregnant rats weighing between 200 to 300gms each, were divided into 5 groups: Group 1(11=10), served as control group. This group comprised normal pregnant rats that didn t receive any injection. Group 11(11=10), served as a model ofuntreated preeclamptic rats, as these rats were injected with L NAME for induction ofpreeclampsia, in a dose 50mg/kg, intraperitoneal (I.P), daily fo r 5 days starting fro m 151 day of pregnan . Group III (11=30), in which the antioxidant vitamin E was injected I.M. in 3 different doses (300, 600, 900 mg/kg), daily f or 5 days before induction of preeclampsia as in group II. Group IV (11=30), in which the antioxidant vitamin C was injected I.M, in 3 different doses (300, 600, 900 mg/kg), daily for 5 days bef ore induction ofpreeclampsia as in group II. Group V(II=10) in which the antioxidant Selenium was injected I.P, in a dose 100llg/day, daily for 5 days bef ore induction ofpreeclampsia as in group II. Rat tail sys tolic blood pressure (S8P) and protein in urine were measured in all groups at 10th , 12th, 14th 17th and 19th days of pregnancy. Results: Preeclamptic rats treated with all doses of vitamin E showed significant reduction of S8 P and improvement of proteinuria on the 191 day ofpregnancy, compared to the sam e day of untreated preeclamptic rats. Comparing the S8P lowering effect of the different doses of vitamin E, it was f ound that the maximum reduction of S8 P was obtained at a dose of vitamin E (600mg/kg),the same dose was also the most effec tive in reducing protein in urine. Preeclamptic rats treated with all doses of vitamin C showed significant reduction of S8 P and improvement ofproteinuria 011 both 171 and 19th days ofpregnancy, compared to the same days of untreated preeclamptic rats. The most ef fective dose of vitamin C on reduction of S8P and improvement of proteinuria was the largest dose (900mg/kg). Preeclamptic rats treated with selenium showed significant reduction of S8P and improvement of proteinuria on both 17th and 19th days ofpregnan , compar ed to the same day of untreated preeclampt ic rats. However. the reduction in S8P achieved by the use of different antioxidants in different doses never reached normal level as that of in normal pregnant rats. Conclusion: The use ofantioxidants call partially correct high S8P and proteinuria and thus reduce the risk of preeclampsia in a dose dependent manner. The maximum reduction in S8P and prot einuria was achieved by the use of vitamin C at the large dose (900mg/kg).
