Author/Authors :
DAS, SANJOY Indian Veterinary Research Institute - Division of Bacteriology and Mycology, India , DAS, SANJOY Central Institute of Fisheries Technology - MFB Division, India , SINGH, V.P. Indian Veterinary Research Institute - Division of Bacteriology and Mycology, India , LTU, KEDUZOL Indian Veterinary Research Institute - Division of Bacteriology and Mycology, India , KATHIRESAN, S. Indian Veterinary Research Institute - Division of Bacteriology and Mycology, India , SHARMA, BHASKAR Indian Veterinary Research Institute - Division of Bacteriology and Mycology, India , BHILEGAONKAR, K.N. Indian Veterinary Research Institute - Division of Bacteriology and Mycology, India
Abstract :
Three different methods of processing samples from freshwater fish, prawn and chicken meat were compared with nested PCR for the detection of Listeria monocytogenes. Nested PCR failed to detect the organism from freshwater fish, prawn and chicken meat when processing was done by boiling lysis method, but it could detect the organisms from freshwater fish, prawn and chicken meat up to the spiking level of 10^4, 10^3 and 10^5 cfu.g^-1, respectively while processing of sample was done by phenol extraction without enrichment. However, using phenol extraction after enrichment, the sensitivity was found higher than without enrichment and it was possible to detect up to the spiking level of 10^2, 10 and 10^3 cfu.g^-1 in case of fish, prawn and chicken meat, respectively.
