Isolation and Biomedical Screening of the Tissue Extracts of Two Marine Gastropods Hemifusus pugilinus (Born, 1778) and Natica didyma (Roding, 1798)

The in vitro antibacterial activity of the acetone and methanol extracts of the marine gastropods Hemifusus pugilinus (Born, 1778) and Natica didyma (Roding, 1798) were tested against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Enterobacter aerogenes following standard disc diffusion method. Highest activity (19.3±0.39 mm) was observed in the methanol extract of N. didyma against E. coli. The extracts were also subjected to anti-inflammatory activity using human red blood cell (HRBC) membrane stabilisation method. All the extracts exhibited antiinflammatory activity, and was high in the acetone extract of H. pugilinus (72.58±1.66%) at 1mL concentration. However, the control drug hydrocortisone exhibited significantly higher activity than the tissue extracts of both the animals. The extracts were screened for their alpha amylase inhibitory activity also and all the extracts inhibited the alpha amylase enzyme activity. Among the mollusc extracts, maximum inhibition was observed in H. pugilinus acetone extract at 50 μL concentration (72.23±0.44%). But, the control drug (Acarbose) had significantly higher inhibitory action than the tissue extracts of both the animals. The amount of protein was comparatively high in the tissue of both H. pugilinus (1.34±5.63 μg.mL^-1) and N. didyma (0.90±4.37 μg.mL^-1) than carbohydrates and lipids.