Author/Authors :
TRAN, NGOC TUAN Chinese Academy of Sciences - Institute of Hydrobiology - Key Laboratory of Aquatic Biodiversity and Conservation, China , TRAN, NGOC TUAN Ministry of Education - College of Fisheries - Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, China , TRAN, NGOC TUAN Ministry of Agriculture, Huazhong Agricultural University - Key Lab of Freshwater Animal Breeding, China , LIU, HAN Ministry of Agriculture, Huazhong Agricultural University - Key Lab of Freshwater Animal Breeding, China , LIU, HAN Ministry of Education - College of Fisheries - Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, China , WANG, WEI-MIN Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, China , WANG, WEI-MIN Ministry of Agriculture, Huazhong Agricultural University - Key Lab of Freshwater Animal Breeding, China , WANG, WEI-MIN Ministry of Education - College of Fisheries - Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, China
Abstract :
Interleukin 10 (IL-10) is important in the inflammatory cytokine mechanisms of many organisms. This study describes the identification and characterisation of the IL-10 homolog obtained from transcriptome profile of blunt snout bream Megalobrama amblycephala Yih 1955 (Cyprinidae): MaIL-10. It contained an ORF of 534 bp encoding a putative protein of 177 amino acids. MaIL-10 protein contained a conserved IL10 family domain. Physicochemical characterisation analysis revealed that it was soluble, basic, hydrophilic, unstable based on the instability index, and thermostable based on its aliphatic index. Alpha helix (56.50%) was predominant among the secondary structure elements, followed by random coil (25.42%), extended strand (12.43%) and beta turn (5.65%), while the remaining elements were absent. MaIL-10 3-D model was generated by comparative modelling using a human IL-10-like (PDB ID: 1lqs.1.D) as the template. The acceptable model was validated using Procheck’s Ramachandran plot, ProQ and ProSA-web tools. Immune challenge of blunt snout bream with Aeromonas hydrophila bacterium (1.8×10^5 cfu fish^-1) demonstrated a significant differences in the expression of maIL-10 in liver, spleen and kidney within 120 h post-infection (P 0.01). The results of this study indicate that maIL-10 plays an important role in the innate immune system of blunt snout bream.
