Author/Authors :
Mahmood, B , Higgs, N University of Manchester - School of Medicine - Deptt of Medicine, UK , Howl, L University of Manchester - School of Medicine - Deptt of Medicine, UK , Warhurst, G University of Manchester - School of Medicine - Deptt of Medicine, UK.
Abstract :
Recent studies primarily in man have shown that cod fish oil rich in polyunsaturated fatty acids (PUFA) are beneficial to certain inflammatory diseases such as ulcerative colitis. This study was undertaken to observe any change in intestinal secretion where the tissues have been treated with cod fish and sunflower oils. Male Sprague Dawley rats weighing 200 gms were fed omega-3fatty acids for 50 days. Changes in colonic secretion (fed with PUFA) were studied in-vitro in an Ussing chamber. Rat colon which were not fed with PUFA served as controls. Basal intestinal short circuit in PUFA group were comparable with control group in stripped rat colon. The results showed significant high short circuit current in cod fish oil and sunflower oil treated tissues. When stripped colonic tissues (fed with PUFA) were stimulated by ECso of carbachol, bradykinin and prostaglandin; there was no significant changes in Short circuit current. PGE2 and LTB-t levels were measured in rat colon fed with PUFA by using radioimmunoassay. Biochemical changes in PGE2 and LTB41eveis showed LTB4 were significantly raised in both cod fish oil group and sunflower oil group. This study reveals that Intesfirral permeability increases in the rat colon (fed with PUFA) as indicated by high short circuit current. The high levels of leukotriene in colonic tissues also explains the high basal short circuit current in the present study.
