Author/Authors :
Rweish, Asia J. AL-Nahrain University - Biotechnology Research Center, Iraq , Ibrahim, Mohammed A. K. AL-Nahrain University - Biotechnology department, Iraq , Abdul-karim, Rafid A. Baghdad University - AL-Khwarzimi College, Iraq , Abdul-majeed, Mohammed R. AL-Nahrain University - Baghdad /Iraq - Biotechnology department, |Iraq , Jawad, Zahra K. AL-Nahrain University - Biotechnology Research Center, Iraq
Abstract :
Ethyl acetate extracts of fermentation cultures of five local Streptomyces isolates SRY-3, SRY-25, 5b, 10, NS-38 were identified as inhibitors of plasmacytoma cell line. Concentration at 0.016 μg/ml of ethyl acetate extract of (SRY-3,SRY- 25,5b,10,NS-38) inhibited (46%,40%,21%,34%,34%) of mouse plasmacytoma cells respectively, where as 0.5 μg/ml inhibited (58%, 55%, 35%, 33%, 36%) of plasmacytoma cells . Quantities analysis of crude extracts by using HPLC on the basis of their retention times showed that the values were (4.3,4.4,4.86,4.83 4.84) min. for SRY-3, SRY-25, 5b, 10,NS-38 respectively, while the retention time of standard antitumor compounds was (4.26) min. This suggests that unknown compound of SRY-3 extract contain bleomycin-like compound. Addition of standard bleomycin to crude extract of SRY-3 increased milliabsorbans unit (M.A.U.) from 0.49 to 1.4. The concentration of bleomycin-like antitumor in fermentation broth of SRY-3 isolate was 3.441 μg/ml. The five Streptomyces isolates have moderate activity against gram-positive and gram-negative bacteria, while standard BLM have no activity.
