The ability of polyphenols extracted from green tea Camellia sinensis in influence of genetic cytotoxicity of catechol on mice bone marrow cells ( in vivo )

Aqueous and methanolic of polyphenols and trepenoids extractions were obtained from green tea leaves. Process of confirmation were performed with conventional qualitative chemical tests,polyphenols(F1)and trepenoids(F2) represented 27.6% and 3.0% from dry weight of leaves recepectively .Catechins recovery was 67.2% from the original polyphenols (F1) contents by HPLC. Results showed that, a single oral administration for mice of both catechol concentrations (156 and 234 mg/kg) caused decreasing mitotic index (MI), blastogenic index (BI) and increasing micronucleus frequency (MN) in bone marrow, catechins treatment due to non significant changes in BI, significant increasing in MI and decreasing in MN, while the combination treatments (catechins and catechol) improved the cytogenetic parameters (BI, MN) in comparison with single treatments of catechol. In fact a clear effect in mitotic activity was reveal such as decreasing in MI and significant increasing in MN after Methotrexate (MTX) treatment dependent on the periods of administration (2-6 weeks), and/or in comparison with negative control.