Detection of Urovirulence Genes (eae,E-hly,α-hly) of Uropathogenic Escherichia coli by Specific PCR

Urinary tracts infection remains a common troublesome health problem in the world. Although E. coli is normal intestinal flora but considered as the main important opportunistic active uropathogene because of its pathogenicity which referred to it’s different virulence factors like hemolysin, biofilm, enterotoxins, shiga like toxins, cytotoxic necrotizing factor and others. Seventy seven E. coli isolates which isolated from 125 midstream urine samples of patients suffering from different types of UTIs. The results showed that 44 isolates 57% were produced hemolysin. These isolates were differed in the efficiency of erythrocyte lysis because which depend on type of hemolysin and source of blood. Seventy two isolates 90.9% were formed biofilm with variety in thickness of biofilm layer. Each isolate produce hemolysin also formed biofilm. Presence of genes encoded for virulence traits of UPEC (hemolysin, biofilm) were examined by PCR with specific primers. The results showed that the percentage of α-hly gene encoded for α-hemolysin 50% while percentage of eae gene encoded for intimin and E-hly gene encoded for enterohemolysin were 40% and 20% respectively.