In vitro and in vivo, antibacterial effect of lactobacillus and ethanolic extract of sage Salvia officinalis on enteropathogenic E.coli (EPEC)

The antibacterial effect of Lactobacillus cell-free filtrate and ethanolic extract of sage Salvia officinalis on enteropathogenic E.coli (EPEC) were investigated in vitro and in vivo. In vitro, antibacterial activity of Lactobacillus and ethanolic extract of sage were determined by using well diffusion method. The results of ethanolic extract of sage showed moderate antibacterial activity even with high concentration of extract 20mg/ml with maximum inhibition zone 18mm. while, of Lactobacillus cell-free filtrate presented high antibacterial activity against E.coli (24mm) in vivo, thirty two albino male mice(age 8-10 weeks, weight ranged 23-27 gram) were used in this experiment. The animals were divided into four equal groups, include positive and negative controls. Histological analysis of intestine, liver and kidney showed that the mice infected with EPEC induce attaching and effacing (A/E) lesions and loss of microvillus actins rootlets as well as microvillus fragmentation and no significant changes in liver and kidney tissues of mice infected with EPEC. Normal microvilli and mucosal morphology observed in mice infected and treated with Lactobacillus and ethanolic extract of sage.