تشخيص بعض انواع الـ Archaeaمن مياه الينابيع الحارة في حمام العليل بمدينة الموصل بالاعتماد على الصفات المظهرية والفحوصات الايضية و تفاعل البلمرة المتسلسل PCR

Isolate, Identify of some species belonging to Archaea from water of hot and sulphid springs in Hammam Al-Allel City/ Nenavah Governorate. Seventy five samples were collected from hot springs during 2012. The Archaea species were isolated using ordinary and selective media then identified according to morphological characters such as shape, colour, consistency of colonies,The isolated Archaeal species showed optimum growth at temperatures between 37-40 C°, while Halobacterium spp. grown at 30-50 C°, and Sulfolobus spp. between 35-70 C°. and when grown in different levels of pH. The results showed the ability of Halobacterium spp. to grow at pH between 4-7 and optimum growth at 3.5-4.5, while Sulfolobus spp.was shown good growth at 3-7 and optimum growth at 3-4, when grown in different concentrations of sodium chloride.The results showed that Halobacterium spp.were grow well in concentrations between 4.5-5.5% while Sulfolobus spp.were grown in concentrations between 2-3%. PCR technique based on amplification of genomic DNA using Specific primers for Archaeal species, was used to confirm the identification of Archaeal and the result indicated one distinct band with molecular size of 930-950 bp in comparison with DNA ladder after electrophoresis on agaros gel.The result of identification showed three species of Archaea in these studied springs, Sulfolobus solfataricus, Sulfolobus acidocaldarius, Halobacterium salinarum.