Antimicrobial Effect of Silver Nanoparticles Synthesized with Bougainvillea Glabra Extract on Staphylococcus Aureus and Escherichia Coli

Considering the antimicrobial properties of silver and its enhanced level at nanoscale scale, it can be used to combat the various pathogens and microbial agents. The aim of this study was to investigate the antimicrobial effect of silver nanoparticles synthesized with Bougainvillea Glabra extract on standard strains of Staphylococcus aureus and Escherichia coli. In this study, silver nanoparticles were biosynthesized using the aquatic Bougainvillea Glabra extract under optimal conditions. The synthesis of silver nanoparticles was confirmed using UltraViolet-Visible (UV-Vis) spectroscopy and X-Ray Diffraction (XRD). Based on the X-ray diffraction pattern, the silver nanoparticles crystallite size was 21 nanometers. Transmission Electron Microscopes (TEMs) and Scanning Electron Microscopes (SEM) showed the synthesis of silver nanoparticles of about 23 nm in size and spherical morphology. Revitalizing and stabilizing agent groups were identified using Fourier-Transform InfraRed (FT-IR) spectroscopy. The mean diameter of the inhibition zone and the Minimum Inhibitory Concentration (MIC) were 27.6 mm and 3.12μg/mL for S. aureus and 19.3mm and 12.5μg/mL for E. coli, respectively. Biological synthesis using Bougainvillea Glabra aquatic extract is a very inexpensive and cost-effective method. The ability of Bougainvillea Glabra to synthesize silver nanoparticles makes it possible to use this plant as a useful biological source for the synthesis of silver nanoparticles with suitable and practical sizes for medical and microbicide applications.