Author/Authors :
kochan, ewa medical university of łódź - pharmaceutical biotechnology department, Poland , caban, sylwia medical university in łódź - pharmaceutical faculty, Poland , szymańska, grażyna medical university of łódź - pharmaceutical biotechnology department, Poland , szymczyk, piotr medical university of łódź - pharmaceutical biotechnology department, Poland , lipert, anna medical university of łódź - department of sport medicine, Poland , kwiatkowski, paweł pomeranian medical university - department of microbiology, immunology and laboratory medicine, poland , sienkiewicz, monika medical university of lodz - department of allergology and respiratory rehabilitation, Poland
Abstract :
Plant suspension cultures are described as a source for the acquisition of medicinal secondary metabolites which in the future may become an alternative to traditional raw materials. This study demonstrates that the cell cultures of one of the ginseng species – Panax quinquefolium L. synthesize ginsenosides, which are triterpene saponins having a multidirectional pharmacological effects. Tested suspension cultures were run on a small scale in the shake lasks and in scale up of the process in a 10-liter stirred tank. In the shake lasks, the highest biomass yield (2.28 gl^-1 for dry and 33.99 gl^-1 for fresh weight) was reached on day 30 of culture, and the highest content of saponins (2.66 mg g^ -1 dw) was determined on day 28 of culture. In the bioreactor, nearly 2.67 and 3-fold increase of respectively dry and fresh biomass was recorded in relation to the inoculum. Large-scale cultures synthesized protopanaxatriol derivatives such as Rg1 and Re ginsenosides, however, no saponins belonging to the protopanaxadiol derivatives were reported.
