Changes of Electrophoretic Protein Profiles of Smoked and MarinatedRainbow Trout (Oncorhynchus mykiss) During Refrigerated Storage

In this study, we aimed to determine the changes of electrophoretic protein profiles of smoked and marinated rainbowtrout (Oncorhynchus mykiss) during refrigerated storage. Changes in muscle proteins during 9 weeks refrigerated storage of raw, smoked and marinated trout samples have been examined using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE and densitometric analysis revealed that intensity and the number of some protein bands were reduced while the bands of actin didn’t change significantly during marination and smoking of rainbow trout muscle. Myosin heavy chain (MHC) band with molecular weight of 205 kDa disappeared in the 9th week of smoking and marinating process. Subsequently, there were significant weekly changes in the protein percentages of samples caused by smoking and marinating processes (P 0.01). Protein percentages of the raw, smoked and marinatedsamples were determined as 18.34-18.82%, 23.83-24.70% and 16.29-17.76%, respectively.