Author/Authors :
Dai, Yong Jinan University - Second Clinical Medical College, Shenzhen People’s Hospital - Clinical Medical Research Center, China , Sui, Weiguo Hospital of Guilin - Kidney Transplantation and Hemo Purification Center, China , Lan, Huijuan Hospital of Guilin - Kidney Transplantation and Hemo Purification Center, China , Yan, Qiang Hospital of Guilin - Kidney Transplantation and Hemo Purification Center, China , Huang, He Hospital of Guilin - Kidney Transplantation and Hemo Purification Center, China , Huang, Yuanshuai Ministry of Education - Chongqing Medical University - Laboratory Medicine, Key Laboratory of the Laboratory Medical Diagnostics, China
Abstract :
Objective: To explore the relationship between immunoglobulin A nephropathy (IgAN) and microRNA (miRNA). Methods: We analyzed the miRNA expression profiles in renal biopsies from 11 IgAN patients and 3 controls at the Kidney Transplantation and Hemo Purification Center of 181 Hospital, China, from May to October 2007, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. Results: This study identified 132 miRNAs in renal samples, of which 31 miRNAs down-regulated and 35 miRNAs up-regulated in IgAN biopsies. The chip results were confirmed by northern blot analysis and by quantitative real-time polymerase chain reaction (RT- PCR) tests. Conclusion: Our study may help clarify the molecular mechanisms involved in the pathogenesis of IgAN, and miRNAs potentially serve as a novel diagnostic biomarker of IgAN.
