Title of article :
A Revisit into the DEN2 NS2B/NS3 Virus Protease Homology Model: Structural Verification and Comparison with Crystal Structure of HCV NS3/4A and DEN2 NS3
Author/Authors :
Keel, Lee Yean university of malaya - Faculty of Science - Department of Chemistry, Malaysia , Othman, Rozana university of malaya - Faculty of Science - Department of Chemistry, Malaysia , Wahab, Habibah A Universiti Sains Malaysia - School of Pharmaceutical Sciences, Malaysia , Yusof, Rohana university of malaya - Faculty of Medicine - Department of Molecular Medicine, Malaysia , Abd. Rahman, Noorsaadah university of malaya - Faculty of Science - Department of Chemistry, Malaysia
Abstract :
Although the crystal structure of DEN2 NS3 serine protease has been reported, the proteolytic mechanism of this enzyme in the presence of NS28 as co-factor which greatly enhances its activity is not well-understood. Using the homology model of DEN2 NS3 co-co rnplexed with NS2 8 co factor based on HCV NS3/4A as the proposed template, the model of the DEN2 NS28 /3 complex was reproduced and its structure evaluated through PROCHECK, VERIFY3D and ERRAT. Comparison of the homology model with the crystal structure of DEN2 NS3 revea led that this homology model served as a better choice for the NS28/3 protein model in drug design.
Keywords :
Dengue , NS3 , serine protease , structural verification , HCV
Journal title :
Malaysian Journal of Science
Journal title :
Malaysian Journal of Science
