Title of article
Hypoglycemic, Insulinotrophic and Cytotoxic Activity of three species of Ganoderma
Author/Authors
Bastami, Mohd Saufi Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Microbiology, Malaysia , Mohd Bohari, Siti Pauliena Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Microbiology, Malaysia , Har, Wong Mee Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Microbiology, Malaysia , Wahab, Mohd. Noor Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Microbiology, Malaysia , Rahmani, Ariffi Suraya Universiti Kebangsaan Malaysia - Faculty of Allied Health Sciences - Department of Biomedical Science, Malaysia , Rajab, Nor Fadilah Universiti Kebangsaan Malaysia - Faculty of Allied Health Sciences - Department of Biomedical Sciences, Malaysia , Hamid, Muhajir Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Microbiology, Malaysia
From page
41
To page
46
Abstract
The crude extracts of Ganoderma lucidum, Ganoderma tropicum and Ganoderma tsugae were studied for insulinotrophic and hypoglycemic activities using rat pancreatic ß-cell lines, BRIN-BD11 cells and normal Wistar rats. In an oral glucose tolerance test (OGTT), oral administration of 1 g/kg body weight of the crude extract resulted in a significant reduction in blood glucose in the normal rats. In acute 30 min insulin secretion tests, 1 mg/ml crude extract of all tested Ganoderma evoked a 1 - 4 fold stimulation of insulin secretion from the pancreatic β-cell lines. Of these, G. lucidum showed the highest insulinotrophic activity. In addition, cytotoxicity assay of extracts from G. lucidum, G. tropicum and G. tsugae towards rat pancreatic β-cell lines showed half maximal inhibition (IC50) at 200, 100 and 150µg/ml, respectively. These results demonstrated the presence of insulin-releasing and hypoglycemic activity in the crude extracts of G. lucidum, G. tropicum and G. tsugae.
Keywords
Ganoderma , hypoglycemic , insulinotrophic , rat pancreatic β , cell lines
Journal title
Malaysian Journal of Science
Journal title
Malaysian Journal of Science
Record number
2680241
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