Title of article :
The effect of silver nitrate, chloroformic garlic extract, and normal saline in induction of sclerosing cholangitis in rabbits
Author/Authors :
Hosseini, V. Nemazee Hospital - Gasteroenterohepathology Research Center - Departments of Surgery, ايران , Mohebzadeh, Jalil Nemazee Hospital - Gasteroenterohepathology Research Center - Departments of Surgery, ايران , Kumar, Perikala V. shiraz university of medical sciences - School of Medicine - Department of Pathology, شيراز, ايران , Mehrabani, Davood Nemazee Hospital - Gasteroenterohepathology Research Center - Departments of Surgery, ايران , Bagheri, Mohammad H. shiraz university of medical sciences - School of Medicine - Department of Radiology, شيراز, ايران , Sadjjadi, M. shiraz university of medical sciences - School of Medicine - Department of Parasitology and Mycology, شيراز, ايران , Amini, Masoud Nemazee Hospital - Gasteroenterohepathology Research Center - Departments of Surgery, ايران , Amini, Afshin
Abstract :
Objectives: To evaluate the effects of 0.5% silver nitrate, 20% chloroformic garlic extract, and 0.9% normal saline in induction of sclerosing cholangitis in the bile ducts of rabbits.Methods: During a-6-months period from April to September 2006 in Shiraz University Laboratory Animal Research Center, we selected 3 equal groups of rabbits. We injected 0.5% silver nitrate, 20% chloroformic garlic extract, and 0.9% normal saline into the bile ducts of each group. The animals were euthanized, and autopsied after 4 months and the liver and bile ducts were removed and studied histopathologically. Cholangiography was undertaken to evaluate the presence and extent of any sclerosing cholangitis.Results: Animals showed sclerosing cholangitis in silver nitrate group (7 [58%]), one (8%) in chloroformic garlic extract group and one (7%) in normal saline group. The difference between silver nitrate and chloroformic garlic extract groups were statistically significant and similar results were noticed between chloroformic garlic extract and normal saline groups.Conclusion: Twenty percent of chloroformic garlic extract had fewer complications such as sclerosing cholangitis, compared to other materials
