Clinical and Histological Evaluation of Low Level Laser on Regeneration of Hip Joint Cartilage in Rabbit

Treating articular cartilage in dogs is a challenge in the contemporary orthopedics ofsmall animals. Currently application of Low Level Laser being advocated for variousorthopedics disorders that is why its effect being evaluated in experimentally induceddefect in hip joint. The experiment was conducted on 24 adult male rabbits whichwere divided into two groups of (control experimental) with 12 rabbits each.Effective anesthesia was induced using combination of acepromazine, ketamine andxylazine. After exposure and subluxation of right femoral head, the maximumaccessible cartilage was denuded up to subchondral bone using dental bit in eachrabbit. No treatment was given to control group, whereas experimental one wassubjected to Low Level Laser Therapy daily for 1 minutes diode laser 630 nm and2 minutes of infrared with 890 nm for 14 days daily, then further they weresubdivided into 3 subgroup of 4 rabbits each with duration of 1 ,2 and 3 months.There was no trace of firbrocartilage or cartilage formation in control group sampleseven up to end of observation period .There was remarkable connective tissue withtrace of chondrocytes beneath that which were located in the large lacunae havingdifferent type of isogenic during one month period in experimental group.. Scatteredcollagen fibers between chondrocyte cells was seen which was not enough to fill thedefect in one month period, whereas drastic changes was noticed in two and threemonths duration with full coverage of defect area with newly formed cartilage having well located chondrocytes in a very large lacuna with huge compact collagenfibers.