Author/Authors :
Freisleben, Seruni K. U. university of indonesia - Faculty of Medicine, Faculty of Mathematics and Natural Sciences - Postgraduate Study Program Biomedical Sciences, Department of Physics, Indonesia , Hidayat, Jenny university of indonesia - Faculty of Medicine - Postgraduate Study Program Biomedical Sciences, Indonesia , Freisleben, Hans-Joachim university of indonesia - Faculty of Medicine - Postgraduate Study Program Biomedical Sciences, Indonesia , Poertadji, Soehardjo university of indonesia - Faculty of Mathematics and Natural Sciences - Department of Physics, Indonesia , Kurniawan, Budi university of indonesia - Faculty of Mathematics and Natural Sciences - Department of Physics, Indonesia , Bo, Na Peng. university of indonesia - Faculty of Mathematics and Natural Sciences - Department of Physics, Indonesia , Handayani, Sri university of indonesia - Faculty of Mathematics and Natural Sciences, Faculty of Medicine - Department of Chemistry, Postgraduate Study Program Biomedical Sciences, Indonesia , Wahidiyat, Pustika A. Dr. Cipto Mangunkusumo Hospital - Thalassemia Center - Department of Child Health, Indonesia , Soegianto, Rondang R. university of indonesia - Faculty of Medicine - Department of Biochemistry and Molecular Biology, Indonesia
Abstract :
Background: Over the last 10 years, we have investigated thalassemia patients in Jakarta to obtain a comprehensive picture of iron overload, oxidative stress, and cell damage. Methods: In blood samples from 15 transfusion-dependent patients (group T), 5 non-transfused patients (group N) and 10 controls (group C), plasma lipids and lipoproteins, lipid-soluble vitamin E, malondialdehyde (MDA) and thiol status were measured. Isolated eryhtrocyte membranes were investigated with electron paramagnetic resonance (EPR) spectroscopy using doxyl-stearic acid and maleimido-proxyl spin lables. Data were analyzed statistically with ANOVA. Results: Plasma triglycerides were higher and cholesterol levels were lower in thalassemic patients compared to controls. Vitamin E, group C: 21.8 vs T: 6.2 μmol/L) and reactive thiols (C: 144 vs. T: 61 μmol/L) were considerably lower in transfused patients, who exert clear signs of oxidative stress (MDA, C: 1.96 vs T: 9.2 μmol/L) and of tissue cell damage, i.e., high transaminases plasma levels. Non-transfused thalassemia patients have slight signs of oxidative stress, but no signifi cant indication of cell damage. Erythrocyte membrane parameters from EPR spectroscopy differ considerably between all groups. In transfusion-dependent patients the structure of the erythrocyte membrane and the gradients of polarity and fluidity are destroyed in lipid domains; binding capacity of protein thiols in the membrane is lower and immobilized. Conclusion: In tranfusion-dependent thalassemic patients, plasma lipid pattern and oxidative stress are associated with structural damage of isolated erythrocyte membranes as measured by EPR spectroscopy with lipid and proteinthiol spin labels. (Med J Indones 2011; 20:178-84)
Keywords :
electron paramagnetic resonance spectroscopy , erythrocyte membrane , lipoproteins , oxidative stress , thalassemia , plasma lipids.