Author/Authors :
Kumara, Shikha , Sachdevac, Nikhil Banaras Hindu University - Pharmaceutical Chemistry Research Laboratory, , Institute of Technology - Department of Pharmaceutics, INDIA. , Amir, Mohmad , Kumarb, Amit Centre for Biomedical Engineering - Indian Institute of Technology, INDIA , Singh, Sushil K. Banaras Hindu University - Pharmaceutical Chemistry Research Laboratory, , Institute of Technology - Department of Pharmaceutics, INDIA
Abstract :
The ethanolic extract of Phyllanthus simplex (Euphorbiaceae) was found to contain free and conjugated phenolic compounds and was therefore screened for anti-oxidant activity using in vitro and in vivo experimental models. The extract exhibited a high degree of inhibition on rat brain lipid peroxidation up to 92.01± 0.002% by in vitro method. P. simplex extract also exhibited dose dependent inhibitory effects on lipid peroxidation up to the concentration of 18.7 ìg/ml of brain tissue. In vivo studies using albino rats showed that the plant extract (100mg extract/kg, p.o.) significantly antagonized CCl4 (0.5ml/kg, p.o.) induced increase in malondialdehyde. The in vivo anti-oxidant activity of P. simplex was found to be comparable to that of Vitamin-E (50mg/kg, p.o.), used as standard.
Keywords :
Phyllanthus simplex , Anti , oxidant activity , Lipid peroxidation.
