GENOTOXICITY DETERMINATIONS OF CORIANDER DROP AND EXTRACT OF CORIANDER SATIVUM IN CULTURED FIBROBLAST OF RAT EMBRYO BY COMET ASSAY

The single cell gel electrophoresis (SCGE) or comet assay is a quick, simple, and sensitive technique for measuring DNA damage in cell nucleus. It is well known that medicinal herbs play an important role in the life of human beings, thus it is essential to determine their safety as public health is concerned. In this study the genotoxicity of Coriander drop, herbal pharmaceutical product, and the extract of Coriander sativum were examined in cultured fibroblast of rat embryo using comet assay. The thirteen to fifteen days old rat embryos were lysed with tripsin and after certain steps it was centrifuged and then cultured. After three to five passages, different concentrations (100μl, 200μl, 500μl, and 1000μl) of each product were applied to the fibroblasts. Lysing, electrophoresis, neutralization and staining were carried out. Finally the slides were analyzed with fluorescence microscope. In the test groups the results indicated that Coriander drop at different doses showed some fragmentation of DNA but this damage as a result was deemed to be not significant. However in the case of Coriander sativum extract the results showed no mutagenic effects in comparison with the positive control group (p 0.05). In conclusion, these herbal products did not show any mutagenic effect according to our test, but further genotoxicity assays are recommended.