THE EFFECTS OF ORAL GRAPE SEED EXTRACT ON CISPLATIN-INDUCED CYTOGENOTOXICITY IN MICE

The present investigation was directed to study the possible chemoprotective activity of orally administered grape seed extract (GSE) against cisplatin-induced cytotoxicity and genotoxicity towards mouse somatic and germinal cells in vivo. Pretreatment of mice with GSE (100 mg/kg/day) for 7 days and simultaneously with a single dose of cisplatin (2.2 or 5.5 mg/kg, i.p.) for another day, significantly reduced the frequency of bone marrow micronucleated polychromatic erythrocytes by factors of 1.9 and 1.28, respectively. Furthermore, GSE caused a reduction in bone marrow suppression induced by cisplatin treatment, particularly before the lower dose. In male germline, orally administration of GSE (100 mg/kg/day) for 7 consecutive days before and 7 consecutive days after treatment with a single dose of cisplatin (2.2 or 5.5 mg/kg, i.p.), significantly elevated the levels of sperm motility reduced by cisplatin treatment. Furthermore, GSE significantly decreased the elevated levels of sperm head abnormality induced with cisplatine by factors of 1.6 and 1.2, respectively. Our results indicate that GSE plays a role in attenuating the genotoxicity induced by cisplatin and may provide decreases in the development of secondary malignancy and abnormal reproductive outcomes risks