Title of article :
Identification of Candida Species from Blood Cultures with Fluorescent In Situ Hybridization (FISH), Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Conventional Methods
Author/Authors :
Börekçi, Gülay Mersin University School of Health, Mersin , Ersöz, Gülden Departments of Clinical Microbiology and Infectious Diseases - Medical Faculty of Mersin University, Mersin , Otağ, Feza Departments of Medical Microbiology - Medical Faculty of Mersin University, Mersin , Özturhan, Hakan Departments of Medical Microbiology - Medical Faculty of Mersin University, Mersin , Şen, Sebahat Departments of Medical Microbiology - Medical Faculty of Mersin University, Mersin , Emekdaş, Gürol Departments of Medical Microbiology - Medical Faculty of Mersin University, Mersin , Akalın, Hilal Department of Medical Genetics - Medical Faculty of Erciyes University, Kayseri , Özkul, Yusuf Department of Medical Genetics - Medical Faculty of Erciyes University, Kayseri
Abstract :
Objectives: Rapid and accurate identification of Candida species from blood cultures is crucial to ensure effective antifungal therapy and to reduce the morbidity and mortal-ity associated with bloodstream fungal infections. In this study, we aimed to identify Candida spp. from blood culture samples with fluorescent in situ hybridization (FISH), poly-merase chain reaction-restriction fragment length polymor-phism (PCR-RFLP) and conventional methods. Materials and Methods: A total of 50 yeast positive sam-ples out of 325 blood culture positive samples and 50 blood culture negative samples were examined by FISH, PCR-RFLP and conventional methods to identify Candida spp. Results: All three methods generally were compatible for identification of single-species Candida spp. (p 0.001). But, FISH and PCR-RFLP for the identification of multi-species Candida spp. were found more compatible than conven-tional methods (p 0.001). Besides, FISH is cheaper and quicker than the other two methods in the identification of Candida spp. from blood culture positive samples. The rates of multi-species candidemia with FISH, PCR-RFLP and conventional methods were 20%, 6% and 4%, respectively. Conclusion: Both PCR-RFLP and FISH methods might be preferred for the rapid identification of Candida spp. from blood culture positive samples. However, FISH is a more suit-able method for the detection of multi-species candidemia.
Keywords :
Candida spp. , FISH , PCR , RFLP , Candidemia , identification.
