Author/Authors :
Rouzbahani, Yashar Biomedical Engineering Department - Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran , Kehtari, Mousa Developmental Biology Laboratory - School of Biology - College of Science - University of Tehran, Tehran, Iran , Imani, Mahdi Biomedical Engineering Department - Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran , Saviz, Mehrdad Biomedical Engineering Department - Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran , Shabani, Iman Biomedical Engineering Department - Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran , Moradikhah, Farzad Biomedical Engineering Department - Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran , Alizadeh, Farhad Biomedical Engineering Department - Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran , Karami, Mohammad-Hossein Developmental Biology Laboratory - School of Biology - College of Science - University of Tehran, Tehran, Iran , Seyedjafari, Ehsan Department of Biotechnology - College of Science - University of Tehran, Tehran, Iran , Jooyan, Najmeh Bioelectromagnetics Laboratory - School of Electrical and Computer Engineering - University of Tehran, Tehran, Iran , Mostajabi, Zahra School of Electrical and Computer Engineering - University of Tehran, Tehran, Iran , Faraji-Dana, Reza Center of Excellence on Applied Electromagnetic Systems - School of Electrical & Computer Engineering - College of Engineering - University of Tehran, Tehran, Iran
Abstract :
Weak microwave radiation (WMR) in our environment has raised health concerns in the
public. Among those, communication frequencies are more than ever becoming widespread and their
effects need thorough studies. A correct understanding of these effects in-vivo by in-vitro experiments
shall preferentially use primary cells. In this study we compared non-modulated (CW) and modulated
WMR exposure of biological cells in-vitro. Human ADMSC (Adipose-Derived Mesenchymal Stem
Cells) were exposed to very weak non-thermal levels of microwave Electromagnetic fields at 1135
MHz ,SAR (Specific Absorption Rate) ≈ 0.002 W/kg (Watt per Kilogram) for 30 minutes daily for 4
days. A statistically significant decrease in proliferation rate of these stem cells was observed compared
to the control group with no exposure. When amplitude-modulated exposure (15 Hz (Hertz) with a
depth of 80%) was used with the same carrier frequency of 1135 MHz (Mega Hertz) and consistent
average power, the cell numbers showed no statistically significant difference from the non-modulated
exposure, but were nevertheless lower than the not-exposed control. The observed decrease in
proliferation in response to -weak microwavefields supports the hypothesis that non-excitable cells,
such as undifferentiated mesenchymal stem cells can interact with, and respond to weak electromagnetic
radiation at communication frequencies. Possible mechanisms responsible for the observed results have
been hypothesized and directions provided for future research.
Keywords :
Microwave EM fields , Stem cell , Interaction , weak field , non-thermal effects , Proliferation
