Title of article :
Efficacy of Phage-Antibiotic Combinations Against Multidrug-Resistant Klebsiella pneumoniae Clinical Isolates
Author/Authors :
Qurat-ul-Ain ، Hafiza Department of Microbiology, Faisalabad - Government College University Faisalabad , Ijaz ، Muhammad Department of Microbiology, Faisalabad - Government College University Faisalabad , Siddique ، Abu Baker Department of Microbiology, Faisalabad - Government College University Faisalabad , Muzammil ، Saima Department of Microbiology, Faisalabad - Government College University Faisalabad , Shafique ، Muhammad Department of Microbiology, Faisalabad - Government College University Faisalabad , Rasool ، Muhammad Hidayat Department of Microbiology, Faisalabad - Government College University Faisalabad , Almatroudi ، Ahmad Department of Medical Laboratories - College of Applied Medical Sciences - Qassim University , Khurshid ، Mohsin Department of Microbiology, Faisalabad - Government College University Faisalabad , Chaudhry ، Tamoor Hamid Department of Microbiology, Faisalabad - Government College University Faisalabad , Aslam ، Bilal Department of Microbiology, Faisalabad - Government College University Faisalabad
Abstract :
Background: Increasing antibiotic resistance warrants therapeutic alternatives to eradicate resistant bacteria. Combined phage-antibiotic therapy is a promising approach for eliminating bacterial infections and limiting the evolution of therapy-resistant diseases. Objectives: In the present study, we evaluated the effects of combinations of bacteriophages and antibiotics against multidrug-resistant (MDR) Klebsiella pneumoniae. Methods: Two MDR strains (GenBank no. MF953600 MF953599) of K. pneumoniae were used. Bacteriophages were isolated from hospital sewage samples by employing a double agar overlay assay and identified by transmission electron microscopy. For further characterization of bacteriophages, the killing assay and host range test were performed. To assess therapeutic efficacy, phages (7.5 × 104 PFU/mL) were used in combination with various antibiotics. Results: The phage-cefepime and tetracycline combinations displayed promising therapeutic effects, restricting the growth of K. pneumoniae isolates, as evidenced by recording OD650nm values. Conclusions: The results of the current study showed that phage-antibiotic combination was a potential therapeutic approach to treat the infections caused by MDR K. pneumoniae.
Keywords :
Bacteriophages , Klebsiella pneumonia , Tetracycline , ESKAPE , MDR
Journal title :
Jundishapur Journal of Microbiology (JJM)
Journal title :
Jundishapur Journal of Microbiology (JJM)
