Comparative Detection of Bovine Herpesvirus-1 Infection in Cattle by Enzyme-Linked Immunosorbent Assay and Micro Neutralisation Test

Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpesvirus-1 (BHV-1) that shows a worldwide distribution and results in severe economic losses in the cattle industry. The aims of this study were to; detect the antibodies against the BHV-1 in cattle blood sera samples by the Enzyme-Linked Immunosorbent Assay (ELISA) and micro Neutralisation Test (mNT) and determine the specifity and sensitivity values of these serologic diagnostic tests. Blood samples were randomly collected from 301 cattle (unvaccinated for BHV-1) from the private farms located in Konya. The ELISA and mNT tests were used for detection of antibodies aganist the BHV-1. The Colorado reference strain of BHV-1 was used for the titration and mNT tests. The virus was propagated and titrated in the permanent Madin-Darby Bovine Kidney (MDBK) cells. Twenty-six (8.6 %) sera samples were detected positive by the ELISA applied for the detection of antibodies aganist the BHV-1. 22 (7.3 %) of totaly 301 cattle blood samples for antibodies to the BHV-1 as seropositive by the mNT. Serum Neutralisation 50 (SN50) values of positive samples ranged between 1/1.7 and 1/31.6. In conclusion, the sensitivity and spesifity of tests were detected by the values obtained and their advantages were compared. For the ELISA, the sensitivity and specifity were determined as 100 % and 98 %, respectively while the corresponding values were 84 % and 100 % for the mNT.