Characteristics studies of anti total PSA antibody s binding with prostate

Two groups of uterus tumors (benign and malignant) postmenopausal patients were used to investigate the presence of prostate specific antigen (PSA). Preliminary experiments were performed to follow the binding of 121-anti total PSA antibody with PSA in uterus tissues homogenates of the two groups with their corresponding antigen and found to be(8.8,7.1%) for benign and malignant tumors respectively. An ImmunoRadioMetricAssay (IRMA) procedure was developed for measuring PSA in benign and malignant uterus tumors homogenates. The optimum conditions of the binding of 'l-anti iotal PSA antibody with PSA were as follows: PSA concentration (150,200 ug protein), tracer antibody concentration (125,250 ug protein), pH (7.6,7.2), temp (15,25°C) and time (1.5 hrs) for postmenopausal benign and malignant uterus tumors tissue homogenates, respectively. The use of different concentrations of Na* and Mgions were shown to cause an increase in the binding al concentration of (125,75 mm) of Na' ions and (75,225 mM) of Mg?*ions for benign and malignant uterus tumors homogenates respectively, while the use of different concentrations of urea and polyethylene glycol (PEG) caused a decrease in the binding with the increase in the concentration of each of urea and PEG in thc both cases.