Title of article :
Cytochrome oxidase subunit I (COI) revealed differentiation among populations of Habrobracon hebetor collected from various regions of Iran
Author/Authors :
Koohpayma, Fatemeh Department of Entomology - Jahrom Branch - Islamic Azad University - Jahrom, Iran , Bagheri, Abdoolnabi Plant Protection Research Department - Hormozgan Agricultural and Natural Resources Research and Education Center - Agricultural Research Education and Extension Organization (AREEO) - Bandar Abbas, Iran , Fallahzadeh, Majid Department of Entomology - Jahrom Branch - Islamic Azad University - Jahrom, Iran , Askari-Seyahooei, Majeed Plant Protection Research Department - Hormozgan Agricultural and Natural Resources Research and Education Center - Agricultural Research Education and Extension Organization (AREEO) - Bandar Abbas, Iran , Fathipour, Yaghoub Department of Entomology - Faculty of Agriculture - Tarbiat Modares University - Tehran, Iran , Dousti, Abu Fazel Department of Entomology - Jahrom Branch - Islamic Azad University - Jahrom, Iran
Abstract :
Habrobracon hebetor Say (Hymenoptera: Braconidae) is an
ectoparasitoid wasp in the family Braconidae and is widely used in biological pest
control. Little information is available on the genetic diversity of geographically
isolated populations of H. hebetor. In the present study, we assess the genetic
structure and diversity of geographically distinct populations of H. hebetor
collected from different regions of Iran. To this end, 19 populations of H. hebetor
(Dehloran, Hamadan, Minab, Rudan, Ahvaz, Sari, Semnan, Bandar Lengeh, Haji
Abbad, Jiroft, Shiraz, Sarpol-e Zahab, Gorgan, Isfahan, Urmia, Kahurestan,
Taziyan, Isin, and Sarkhun) were collected from natural niches. For each
population, we sequenced a ~660 base pair fragment of Cytochrome Oxidase
subunit I (COI) successfully. Analysis of molecular variance revealed sharp
differentiation among H. hebetor populations. Populations from Ahvaz, Dehloran,
Jiroft and Minab were the most genetically diverged. A Mantel test showed
significant positive correlation between genetic and geographic distances (r = 0.47,
P < 0.001). The phylogenetic analysis clustered the populations into two major
groups (A and B) (100); the major part was assigned to group A. Group B mainly
included the populations from southern Iran. Based on these results, we conclude
that H. hebetor in Iran is comprised of many diverse populations. These may be
successfully applied in innundative release programs.
Keywords :
Genetic structure , various regions , Haplotype diversity , Bayesian inference
Journal title :
Journal of Crop Protection