Gel clot assay used for detection of candida spp infection in urinary tract

The study including isolation and identification of Candida spp. causing UTIs from patients coming to Al- Yarmouk hospital. A local diagnostic kit was used for measuring canditoxin activity in the urine of patients and it s relation to Candida spp. urine samples were collected from female patients of different ages with UTI. The percentage of C albicans was (26%); C tropicalis was (16%). The results showed a high significant difference between them (p=0.00, chi=21.0). The study comprised and attempts to prepare a local diagnostic kit from the lysate of Homarus lobster, used to indicate the presence of canditoxin and measure it s activity also in urine samples. The study showed a significant increase in canditoxin activity in UTI patients, (69.2%) for C albicans, (75%) for C tropicalis while the percentage of the patients who have no fungal growth was 17.24%. The results showed that the percentage of positive LAL assay in-patients diagnosed with fungal infection was (40%) as compared with negative results (60%).