Title of article
Detection of RAF fusion transcripts in FFPE samples of Medullablastoma and Ependymom in Iraqi children with RT-RQPCR assays
Author/Authors
al obaidy, luma h. a. iraqi centre for cancer research and medical genetics, Iraq , al anssary, nada a. guy’s and st. thomas nhs foundation trust - molecular oncology unit, gsts pathology, UK , al rekabi, nahi y. iraqi centre for cancer research and medical genetics, Iraq , mohammed, haider l. neurosurgery hospital, Iraq , tobal, khalid a. guy’s and st. thomas nhs foundation trust - molecular oncology unit, gsts pathology, UK
From page
1411
To page
1419
Abstract
Medulloblastomas and ependymomas are the most common malignant brain tumors in children. However genetic abnormalities associated with their development and prognosis remain unclear. Recently two gene fusions, KIAA1549–BRAF and SRGAP3–RAF1 have been detected in a number of brain tumours. We report here our development and validation of RT-RQPCR assays to detect various isoforms of these two fusion genes in formalin fixed paraffin embedded (FFPE) tissues of medulloblastoma and ependymoma. We examined these fusion genes in 44 paediatric brain tumours, 33 medulloblastomas and 11 ependymomas. We detected both fusion transcripts in 8/33, 5/33 SRGAP3 ex10/RAF1 ex10, and 3/33 KIAA1549 ex16/BRAF ex9, meduloblastomas but none in the 11 ependymomas examined. This investigation provided evidence to the value of RT-RQPCR assays for the detection of these fusion genes in large-scale studies on FFPE tissues. The study also reports the first detection of RAF fusion genes in meduloblstomas
Keywords
Medulloblastoma , ependymoma , RAF Gene , RT , RQPCR technique
Journal title
Baghdad Science Journal
Journal title
Baghdad Science Journal
Record number
2690534
Link To Document