Title of article
Separation and Determination of Some Organic Acids in Dry Calyces of Iraqi Hibiscus Sabdariffa Linn
Author/Authors
brahim, sarah k.i university of baghdad - college of science for women - department of chemistry, Baghdad, Iraq , hasan, berween a. ministry of education, Iraq , khalaf, kareem d. university of baghdad - college of science for women - department of chemistry, baghdad, Iraq
From page
340
To page
349
Abstract
A new reversed phase- high performance liquid chromatographic (RP-HPLC) method with Ultraviolet-Visible spectrophotometry has been optimized and validated for the simultaneous extraction and determination of organic acids present in Iraqi calyces of Hibiscus Sabdraffia Linn. The method is based on using ultrasonic bath for extracting organic acids. Limit of detection in μg/ml of Formic acid, Acetic acid, Oxalic acid, Citric acid, Succinic acid, Tartaric acid, and Malic acid 126.8498×10-6, 113.6005×10-6, 97.0513×10-6, 49.7925×10-6, 84.0753×10-6, 92.6551×10-6, and 106.1633×10-6 ,respectively. The concentration of organic acids found in dry spacemen of calyces of Iraqi Hibiscus Sabdraffia Linn. under study: Formic acid, Acetic acid, Oxalic acid, Citric acid, Succinic acid, Tartaric acid, and Malic acid are 114.896 μg/g, 64.722 μg/g, 342.508 μg/g, 126.902 μg/g, 449.91 μg/g, 268.52 μg/g, and 254.07 μg/g respectively.
Keywords
Organic acids , Hibiscus Sabdraffia Linn. , RPHPLC , Uv , Vis
Journal title
Baghdad Science Journal
Journal title
Baghdad Science Journal
Record number
2691627
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