Author/Authors :
Rhatomy, Sholahuddin Faculty of Medicine - Universitas Airlangga, Surabaya, Indonesia , Novembri Utomo, Dwikora Faculty of Medicine - Universitas Airlangga, Surabaya, Indonesia , Rosita Sigit Prakoeswa, Cita Faculty of Medicine - Universitas Airlangga, Surabaya, Indonesia , Rantam, Fedik Abdul Faculty of Veterinary Medicine - Universitas Airlangga, Surabaya Indonesia , Suroto, Heri Faculty of Medicine - Universitas Airlangga, Surabaya, Indonesia , Mahyudin, Ferdiansyah Faculty of Medicine - Universitas Airlangga, Surabaya, Indonesia
Abstract :
The hypoxic environment is a substantial factor in maintenance, proliferation, and differentiation
of the cell cultures. Low oxygen is known as a potent chondrogenesis stimulus in stem cells
that is important for clinical application and engineering of functional cartilage. Hypoxia can
potentially induce angiogenesis process by secretion of cytokines. This systematic review goal is
to discover the effect of hypoxic condition on tendon/ ligament culture and the best oxygen level
of hypoxia for in vitro and in vivo studies. We included 21 articles. A comprehensive review
of this database confirms that the hypoxic condition is a substantial factor in the maintenance,
proliferation, and differentiation of ligament/tendon cultures. Cell proliferation in the severe
hypoxic (oxygen concentration of 1%) group at 24 hours post-cultivation was considered
significant, but cell proliferation was markedly inhibited in the severe hypoxic group after 48 h.
Keywords :
Hypoxic condition , Ligament , Tendon , Culture
